Feasibility of A Novel On-Site Detection Method for Aflatoxin in Maize Flour from Markets and Selected Households in Kampala, Uganda

Feasibility of A Novel On-Site Detection Method for Aflatoxin in Maize Flour from Markets and Selected Households in Kampala, Uganda

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In sub-Saharan Africa, there is a high demand for affordable and accessible methods for tokidoki x sanrio blind box on-site detection of aflatoxins for appropriate food safety management.In this study, we validated an electrochemical immunosensor device by the on-site detection of 60 maize flour samples from six markets and 72 samples from households in Kampala.The immunosensor was successfully validated with a linear range from 0.7 ± 0.1 to 11 ± 0.

3 µg/kg and limit of detection (LOD) of 0.7 µg/kg.The maize flour samples from the markets had a mean total aflatoxin concentration of 7.6 ± 2.3 µg/kg with approximately 20% of the samples higher than 10 µg/kg, which is the maximum acceptable bee by bonnie and neil level in East Africa.

Further down the distribution chain, at the household level, approximately 45% of the total number contained total aflatoxin levels higher than the acceptable limit.The on-site detection method correlated well with the established laboratory-based HPLC and ELISA-detection methods for aflatoxin B1 with the correlation coefficients of 0.94 and 0.98, respectively.This study shows the feasibility of a novel on-site detection method and articulates the severity of aflatoxin contamination in Uganda.